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High Throughput Screening(HTS) |
MEKi kinase assay |
| mTORi kinase assay | |
| Histone methyltransferase EHMT2/G9a assay | |
| Lantha EphB4 activity assay | |
| Deubiquitination activity assay | |
| GPCR Modulating activity screen (cell-based) | |
| Lethality of NCI-60 cancer cell panel | |
| Cancer cell lethality screen | |
| PDX-derived primary lethality screen | |
| Cancer stem cell lethality imaging in mix-culture system | |
| PD-1/PD-L1 Bl1ock assay | |
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High Content Screening (HCS) |
Cell viability assay 3D culture system |
| Apoptosis assay | |
| Translocation activity of target molecules | |
| Detection of changing cell organelles | |
| Detection of DNA damage assay | |
| Cancer cell lethality imaging screen | |
| Primary lethality imaging screen | |
| Cancer stem cell lethality imaging in mix-culture system |
Service
- Phenotype/target-based screening setup → HTS campaign → Time saving in drug development
- Automated/optimized efficacy test for novel anti-cancer drug
- In vitro efficacy test for drug using NCI-60 cancer cell panel
- Selection of hit compound or target cancer
Target enzyme / Phenotype-based screen
Anti-cancer drug in in vitro efficacy test automatization/optimization
Service example
- Screening of apoptosis inducing drugs based on high-throughput screening
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Exam Overview |
- Using image analysis screening, images of caspase-3 activation were analyzed to determine the ability of the developed drug to induce cancer cell death. |
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Test contents |
- Automatic seeding of test cells - Material handling and washing/cell fixing - Stain with cleaved caspase-3 antibody and image with HCS equipment |
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Result |
- Cleavage of caspase-3 occurs due to the activity of the candidate anticancer substance, which is interpreted as cell death. - It was confirmed that the substance had an excellent ability to induce apoptosis compared to the DMSO control and that death occurred more easily in Huh7 cells.  |